Intermediates of Bacteriophage MS2 Assembly In Vivo
نویسندگان
چکیده
منابع مشابه
Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2
Using cross-linking coupled to matrix-assisted laser desorption/ionization mass spectrometry and CLIP-Seq sequencing, we determined the peptide and oligonucleotide sequences at the interfaces between the capsid proteins and the genomic RNA of bacteriophage MS2. The results suggest that the same coat protein (CP)-RNA and maturation protein (MP)-RNA interfaces are used in every viral particle. Th...
متن کاملMolecular interactions in the RNA bacteriophage MS2.
The simple RNA bacteriophages of E.coli have long been used as ideal model systems in which the details of RNA replication, translation and the control of gene expression can be studied in molecular detail’.’. In particular, the translational repression of the phage replicase cistron by coat protein subunits has attracted a great deal of attention and this has become a paradigm for sequence-spe...
متن کاملThe allosteric switching mechanism in bacteriophage MS2.
We use all-atom simulations to elucidate the mechanisms underlying conformational switching and allostery within the coat protein of the bacteriophage MS2. Assembly of most icosahedral virus capsids requires that the capsid protein adopts different conformations at precise locations within the capsid. It has been shown that a 19 nucleotide stem loop (TR) from the MS2 genome acts as an allosteri...
متن کاملBacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid
Using RNA-coat protein crosslinking we have shown that the principal RNA recognition surface on the interior of infectious MS2 virions overlaps with the known peptides that bind the high affinity translational operator, TR, within the phage genome. The data also reveal the sequences of genomic fragments in contact with the coat protein shell. These show remarkable overlap with previous predicti...
متن کاملEncapsidation of heterologous RNAs by bacteriophage MS2 coat protein.
The RNA bacteriophages of E. coli specifically encapsidate a single copy of the viral genome in a protein shell composed mainly of 180 molecules of coat protein. Coat protein is also a translational repressor and shuts off viral replicase synthesis by interaction with a RNA stem-loop containing the replicase initiation codon. We wondered whether the translational operator also serves as the vir...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1974
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.14.5.1152-1168.1974